The Velocyt from Bennubio:
That means the types of high throughput screening campaigns normally associated with flow cytometry and single cell analysis can now be performed on 3D cellular models!
Large partical flow cytometry
LAB WEIGHING AND SORTING FOR
ORGANOIDS AND SPHEROIDS
Multiplexed single-cell analysis of organoid signalling networks
SOstrop J, Zwiggelaar RT, Terndrup Pedersen M, et al. A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation. Front Cell Dev Biol. 2021;8:618552. Published 2021 Jan 21. doi:10.3389/fcell.2020.618552
Rosenbluth, J.M., Schackmann, R.C.J., Gray, G.K. et al. Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages. Nat Commun 11, 1711 (2020). https://doi.org/10.1038/s41467-020-15548-7
Step-by-step dissociation of PSC-derived cerebral organoids into single cells. After dissociation, single cells can be either analyzed by flow cytometry using the MACSQuant® Analyzer 10 or can be cultivated for immunostaining and microscopy analysis.
Hilary Sherman and Hannah J. Gitschier M.S. Corning Incorporated, Life Sciences Kennebunk, ME USA
Kevin Su , Nick Asbrock , Vic Chu, Ph.D. , Stefanie Hoffmann, M.S. , Philip Hewitt, Ph.D
Michael Kowalski, Beckman Coulter Life Sciences, Vipat Raksakulthai, Molecular Devices, Kristin Prasauckas, Molecular Devices, Tara Jones-Roe, Beckman Coulter Life Sciences.
Round up of the latest technology and research