A brief history of organoids.
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Claudia Corrò ,Laura Novellasdemunt, Vivian S.W. LiAm J Physiol Cell Physiol319: C151–C165, 2020.
First published May 27, 2020; doi:10.1152/ajpcell.00120.2020.Licensed under Creative Commons Attribution CC-BY 4.0:©the American Physiological Society. ISSN 0363-6143.http://www.ajpcell.orgC151
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Co-author and
World Organoid Research Day+ Speaker: Diana Papp with Isabelle Hautefort, Martina Poletti and Tamas Korcsmaros. 'In this review, we provide a general introduction for improving the accessibility to these models. We highlight areas for cross-disciplinary collaboration with biomaterial, tissue engineering, and nanofabrication sciences to broaden the application of organoids'.
Engineering organoids.
Everything You Always Wanted to Know About Organoid-Based Models (and Never Dared to Ask)
Engineering organoids.
' In this Review, we argue that many limitations of traditional organoid culture can be addressed by engineering approaches at all levels of organoid systems'. Hofer, M., Lutolf, M.P. Engineering organoids. Nat Rev Mater 6, 402–420 (2021). https://doi.org/10.1038/s41578-021-00279-y
Organoid V Spheroid
What's the difference?
Spheroids
- Simple 3D clusters of single or multiple cell types.
- Do not self-assemble, simply stick together.
- No need for supportive/scaffolding in vitro matrices.
- Cannot regenerate and difficult to long term culture.
- Very successful for disease modelling (including recreating tumours) and stem cell research in Drug Discovery. Inexpensive.
Organoids
- Complex 3D clusters of multiple cells (stem, progenitor) from a specific organ. Self-assemble.
- Require supportive/scaffolding in vitro matrices.
- Form miniature versions of viable parent organs such as mini-gut, mini brain mini lung etc.
- Regenerate, good for long term culture for in vivo like studies.
- Very successful for disease modelling, drug discovery and regenerative medicine. More from Crown Bioscience here
Podcast highlight: Using organoids to transform drug discovery
An Impulse podcast
With Nikolce Gjorevski from Roche
Reporting In Vitro Experiments Responsibly
Reporting In Vitro Experiments Responsibly – the RIVER Recommendations
Publications reporting in vitro experiments often lack basic details that prevent readers from repeating the experiments described or assessing whether the results are reliable. This prevents the experiments adding to the knowledge base and can lead to unnecessary subsequent studies.
Ref: group, The RIVER w. “Reporting in Vitro Experiments Responsibly – the RIVER Recommendations.” MetaArXiv, 20 June 2023.
YouTube Organoid culture videos
Mix of commercial and academic videos for a variety of techniques and models
'Engineering organoids'.
' In this Review, we argue that many limitations of traditional organoid culture can be addressed by engineering approaches at all levels of organoid systems'. Hofer, M., Lutolf, M.P. Engineering organoids. Nat Rev Mater 6, 402–420 (2021). https://doi.org/10.1038/s41578-021-00279-y
Optics11 Life
Topics Covered:
- Relationship between cells & the extracellular matrix (ECM).
- Cell-matrix interactions in organoids, and the therapeutic potential of hydrogels.
- ECM stiffness in disease onset, especially its role in cancer models and the link to fibrosis.
The Brain Organoid HUB, Emory University.
Watch Alexia King speak at World Organoid Research Day 2023
Featured organoid & spheroid 3D guides
Newcells Retinal Organoids eBook
A comprehensive guide discussing iPSC-derived retinal organoids and retinal pigment epithelium for use in in vitro retinal disease modelling and preclinical drug testing.
Register to download.
CN-BIO
Pharmacokinetic profiles revisited in 3D microfluidic tumour models.
Drug metabolism in a gut-liver microphysiological system.
Improved prediction of oral bioavailability using a gut-liver microphysiological system.
Crown Bioscience
eBook: A New Dimension to Drug Discovery
Crown Bioscience has merged our OrganoidBase™ resource with High Content Services (HCS), to provide you with more data, deeper insights, and better results in less time.
AMSBIO
Organoid Culture Handbook.
Reagents | Cells | Matrices
Scroll down page and select.
PhenoVista
PhenoVista allows clients to access the benefits of 3D culture work, without the acquisition of expertise or specialized equipment/culturing capabilities
Cellesce
Organoids: The body builders. Stem-cell-derived organoids.
Open now.
Bennubio
High Throughput Analysis of Spheroid Killing via the Velocyt® Flow Cytometer.
Open here.
OLS OMNI Life Science
The CERO 3D Incubator & Bioreactor allows for simplified scale-up and automation platforms, and cost reduction.
Watch and read now.
CELLENION a BICO Company.
spheroONE® Automated sorting and isolation of single spheroids and organoids. Open here.
MIMETAS
Enhancing 3D Disease Models: Automated, High-throughput Phenotypic Screening with Organ-on-a-Chip
Register to watch webinar
Celldynamics: Introducing a novel Biophysical Characterization for 3D Cell Culture. Discover the journey from the Scientific method to the latest technology. Scroll down to paper.
Latest books
NEW eBook: Exploring Diverse Applications Using Organoid Models
Characterizing and optimizing organoid cultures can be challenging due to a lack of validated protocols for reproducible organoid formation. Another hindrance is the limitations of technologies used for monitoring organoid growth over time.
In this eBook, we explore the value of organoid models to scientific research and discuss the importance of organoid culture quality control (QC). Also featured is how label-free live-cell monitoring and analysis can be used to maximize organoid insights as well as key applications of live-cell imaging technology within the field of organoid research.
Analysis guides and interesting techniques
Genomics: 'Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients..'
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Genomics: 'Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients..'
..are used for a high throughput drug screen to identify gene drug associations that may facilitate personalized therapy. van de Wetering et al., 2015, Cell 161, 933–945 May 7, 2015 ª2015 Elsevier Inc. http://dx.doi.org/10.1016/j.cell.2015.03.053
Intact cytometry: High Throughput Analysis of Spheroid Killing via Velocyt® Flow Cytometry.
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Genomics: 'Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients..'
The Velocyt® revolutionizes 3D cellular analysis by analyzing tens of thousands of spheroids per second while maintaining them in their preferred habitat of liquid cell culture media. In addition, the spheroids are returned undiluted and unchanged allowing for subsequent analysis.
Open now.
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Drug screening and genome editing in human pancreatic cancer organoids identifies drug-gene interactions and candidates for off-label therapy. Hirt et al., 2022, Cell Genomics 2, 100095 February 9, 2022 ª 2022 The Author(s). https://doi.org/10.1016/j.xgen.2022.100095
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids
Genomics: 'Drug screening and genome editing in human pancreatic cancer organoids identifies drug..'
Jourdon A, Scuderi S, Capauto D, Abyzov A, Vaccarino FM. PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids. Neuropsychopharmacology. 2021;46(1):70-85. doi:10.1038/s41386-020-0763-3
Imaging: 'Modelling medulloblastoma in vivo and with human cerebellar organoids'.
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids
Imaging: 'Modelling medulloblastoma in vivo and with human cerebellar organoids'.
Features CrestOptics: Imaging X-Light V2. Published article in Nature on cerebellar organoids. Ballabio, C., Anderle, M., Gianesello, M. et al. Modeling medulloblastoma in vivo and with human cerebellar organoids. Nat Commun 11, 583 (2020). https://doi.org/10.1038/s41467-019-13989-3
Open now.
Large-scale expansion and cryopreservation of human iPSC-based hepatic organoids
PsychENCODE and beyond: transcriptomics and epigenomics of brain development and organoids
Imaging: 'Modelling medulloblastoma in vivo and with human cerebellar organoids'.
Schmidt, K. 1 , Altmaier, S. 1 , Rasmussen M. A. 2 , Meiser, I. 1 , Holst, B. 2 , Bur, S. 1 , Clausen, C. 2 , Nielsen B. S. 2 , Neubauer, J. C. 1,3 , Zimmermann, C. 1,3,4,5 1Fraunhofer Institute for Biomedical Engineering, Sulzbach (GER), 2Bioneer A/S, Hørsholm (DK), 3Fraunhofer Project Center for Stem Cell Process Engineering, Würzburg (GER), 4Saarland University, Saarbrücken (GER), 5Universidad Católica del Norte, Coquimbo (CHL)
Imaging: Present Application and Perspectives of Organoid Imaging Technology
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
Organ-on-a-chip. Main section below but check out this video from Brunel University.
Fei K, Zhang J, Yuan J, Xiao P. Present Application and Perspectives of Organoid Imaging Technology. Bioengineering (Basel). 2022;9(3):121. Published 2022 Mar 16. doi:10.3390/bioengineering9030121
Organ-on-a-chip. Main section below but check out this video from Brunel University.
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
Organ-on-a-chip. Main section below but check out this video from Brunel University.
Organ-on-a-Chip, with Dr Ruth Mackay. Watch now.
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
Transcriptomic Landscape and Functional Characterization of Induced Pluripotent Stem Cell–Derived Cerebral Organoids in Schizophrenia. Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131(5):861-872.
Imaging: LEICA Microsystems
Imaging: LEICA Microsystems
Transcriptomic Landscape and Functional Characterization of iPSC derived cerebral organoids
THUNDER Imager 3D Cell Culture provides you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids. Read and download.
Imaging: OLYMPUS
Imaging: LEICA Microsystems
Imaging: SARTORIUS
Overcome the boundaries of 3D live cell imaging. Register to download.
Imaging: SARTORIUS
Imaging: LEICA Microsystems
Imaging: SARTORIUS
Organoid Analysis Guide.
eBook. Register to download.
Prospective Instruments' MPX
MPX, the world’s first turnkey, compact, fully integrated, next-generation multiphoton microscope!
The MPX combines different imaging techniques in one easy-to-use and portable device: non-linear MP (two-photon, SHG & THG) and widefield epi-fluorescence and fluorescence lifetime imaging to maximize informational content, ranging from single cells up to living animals.
27-28th February 2024
World Organoid Research Day+
In person. Cambridge, U.K
THE EVENT for
Organoid and Organ-on-a-chip researchers.
Free to attend, small donation to charity requested.
Organoid and Organ-on-a-chip talks on both days. WORD+ tickets fund research & research ticket attendees could win £1000 for their lab too.